Starvation induces autophagy of hypertrophic scar fibroblasts (http://www.cjpp.net/EN/abstract/abstract17843.shtml)
［ABSTRACT］AIM: To investigate the starvation-induced autophagy of hypertrophic scar fibroblasts. METHODS: Primary human fibroblasts from hypertrophic scars were isolated and the fibroblasts in logarithmic growth phase were cultured with amino acid-free Earle's balanced salt solution (EBSS) instead of the DMEM medium. The cells were collected at the time points of 0 h, 1 h, 2 h and 3 h after EBSS culture. The expression of microtubule-associated protein 1 light chain 3 (LC-3) and autophagy-related protein Beclin-1 was detected by Western blotting and qRT-PCR. Autopagosomes in fibroblasts were observed by electron microscopy and monodansylcadaverine (MDC) staining. RESULTS: The expression of LC3 and Beclin-1 increased at 1 h after starvation, reached to the highest level at 2 h after starvation, and then began to decline, which were still higher than that in control group. Compared with control group, the autopagosomes were observed in the fibroblasts under fluorescence microscope and election microscope at 2 h after starvation. CONCLUSION: Autophagy in fibroblasts of hypertrophic scars can be induced by starvation, which may be related to the formation of hypertrophic scars.［ABSTRACT］AIM: To investigate the starvation-induced autophagy of hypertrophic scar fibroblasts. METHODS: Primary human fibroblasts from hypertrophic scars were isolated and the fibroblasts in logarithmic growth phase were cultured with amino acid-free Earle's balanced salt solution (EBSS) instead of the DMEM medium. The cells were collected at the time points of 0 h, 1 h, 2 h and 3 h after EBSS culture. The expression of microtubule-associated protein 1 light chain 3 (LC-3) and autophagy-related protein Beclin-1 was detected by Western blotting and qRT-PCR. Autopagosomes in fibroblasts were observed by electron microscopy and monodansylcadaverine (MDC) staining. RESULTS: The expression of LC3 and Beclin-1 increased at 1 h after starvation, reached to the highest level at 2 h after starvation, and then began to decline, which were still higher than that in control group. Compared with control group, the autopagosomes were observed in the fibroblasts under fluorescence microscope and election microscope at 2 h after starvation.
CONCLUSION: Autophagy in fibroblasts of hypertrophic scars can be induced by starvation, which may be related to the formation of hypertrophic scars.
Very interesting. What does starvation refer to in this context?
Yeah I am going to have to reacquaint myself with a few things re cell biology from university then I'll follow up on this one and how it applies to our situation.
I have practiced intermittent fasting (IF) for years.
It did absolutely nothing to my scar.
Your information is very good.
Seems like someone on here was trying this about a year ago? If you give a search may be able to find it.
Might have been me, and that was just fasting. It could be that starvation sufficient to induce autophagy of scar tissues requires far harsher calorie deprivation for much longer periods.